efluor 506 fixable viability dye (Thermo Fisher)

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Thermo Fisher efluor 506 fixable viability dye
Efluor 506 Fixable Viability Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/efluor+506/PBS/pmc12749970-518-7-12
Average 98 stars, based on 1 article reviews

efluor 506 fixable viability dye - by Bioz Stars, 2026-09

98/100 stars

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Staining:

Article Title: Pregnancy enhances antiviral immunity independent of type I IFN but dependent on IL-17–producing γδ + T cells in the nasal mucosa
Article Snippet: .. Cells were initially stained with the eBiosciences fixable viability dye eFluor 506 (Invitrogen, catalog no. 65-0863-18) or eFluor 780 (Thermo Fisher Scientific, catalog no. 65-0865-14) for 30 min at 4°C in PBS and anti-CD16/32 (BD Biosciences; clone 2.4G2) in 1% bovine serum albumin (BSA)/PBS solution to block nonspecific interaction with Fc receptors (10 min; 4°C). .. To identify innate immune cells and epithelial cells, cells were surface-stained (30 min; 4°C) with BV786-conjugated or phycoerythrin (PE)–conjugated anti-SiglecF (BD Biosciences; clone E50-2440); BUV737-conjugated or BUV395-conjugated anti-CD45.2 (BD Biosciences; clone 104); BUV395-conjugated anti-CD11b (BD Biosciences; clone M1/70); peridinin chlorophyll (PerCP)-eFluor710–conjugated anti-Ly6G (BD Biosciences; clone 1A8); allophycocyanin (APC)–conjugated or fluorescein isothiocyanate (FITC)–conjugated anti-Ly6C (BD Biosciences; clone AL-21); PE-Cy7–conjugated, BV786-conjugated, or BV421-conjugated anti-CD11c (BD Biosciences; clone HL3); PeCy7-conjugated anti-MerTK (Thermo Fisher Scientific; clone DS5MMER); PE-conjugated (Thermo Fisher Scientific; clone X54-5/7.1) or PerCP-Cy5.5–conjugated (BioLegend; clone X54-5/7.1) anti-CD64; APC-conjugated anti-F4/80 (Thermo Fisher Scientific; clone BM8); BV650-conjugated anti-NK1.1 (BD Biosciences; clone PK136); and APC-conjugated anti-CD326 (EpCAM) (BD Biosciences; clone G8.8).

Article Title: Generation of an Inhibitory NK Cell Subset by TGF-β1/IL-15 Polarization
Article Snippet: .. Cells were washed twice in FACS buffer (PBS, 2% FBS, 0.05% sodium azide), followed by fixable viability dye eFluor 780 (catalog no. 65-0865-14, 1:1000, eBioscience) or eFluor 506 (catalog no. 65-0866-14, 1:800, eBioscience) stain and FcR blocking Ab (catalog no. 564220, 1:200, BD Biosciences) in PBS at 4°C for 20 min. ..

Article Title: Lung influenza virus-specific memory CD4 T cell location and optimal cytokine production are dependent on interactions with lung antigen-presenting cells
Article Snippet: Cells were first labeled with Fc block for 10 minutes at 4 °C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend, S17007L), CD11b-PeCy7 (BioLegend, M1/70), Ly6G-BV785 (BioLegend, 1A8), Ly6C-PerCP-Cy5.5 (ThermoFisher, HK1.4), MHCII BUV395 (BD, 2G9), CD64-BV711 (BioLegend, X54-5/7.1), CD11c-eFluor780 (ThermoFisher, N418), B220-eFluor 450 (RA3-6B2, ThermoFisher), CD103-Qdot 605 (BioLegend, 2E7), CD3-FITC (145-2C11, BioLegend), CD8α APC (53-6.6, ThermoFisher). .. Cells were then stained with a fixable dye eFluor 506 (ThermoFisher). .. Stained cells washed with FACS buffer or Permwash following intracellular staining and acquired on a BD Fortessa and analyzed using FlowJo (version 10 BD Bioscience, USA).

Article Title: Lung influenza virus-specific memory CD4 T cell location and optimal cytokine production are dependent on interactions with lung antigen-presenting cells
Article Snippet: .. Cells were stained with a fixable viability dye eFluor 506 (ThermoFisher). .. For some experiments, cells were fixed with cytofix/cytoperm (BD Bioscience) for 20 minutes at 4 °C and stained in perm wash buffer with anti-IFNγ PE or BV785 (ThermoFisher or BioLegend, clone: XMG1.2) for 1 hour at room temperature.

Blocking Assay:

Single Cell:

Article Title: IL-1R signaling drives enteric glia-macrophage interactions in colorectal cancer
Article Snippet: Then cells were washed with FACS buffer (0.5% FBS and 2 mM EDTA in PBS) and resuspended in FACS buffer containing the viability marker 7-AAD (1:100 BD Pharmingen) before filtering through a 70-μm strainer. .. For the intracellular measurement of IL-1α and IL-1β, single-cell suspensions were pre-cultured in DMEM with 2.5% FBS, 100 μg/mL Penicillin and Streptomycin and stimulated with BD GolgiStopTM (1:1000, BD Biosciences) for 4 h in 5% CO 2 at 37 °C followed by a pre-incubation with the viability dye eFluor 506 (1:400 eBioscience) for 20 min at 4 °C. .. Then cell suspensions were washed, blocked with FcR Blocking Reagent (1:100 BD Pharmingen) and stained with surface antibodies (see Supplementary Table for antibodies list) as described above.

FACS:

other:

Article Title: Unraveling an enhancer-silencer regulatory element showing epistatic interaction with a variant that escaped genome-wide association studies
Article Snippet: eFluor 506 , ThermoFisher , Cat#65-0866-18.

Incubation:

Article Title: Merkel cell carcinoma-derived macrophage migration inhibitory factor (MIF) may promote persistence of Chronic Lymphocytic Leukemia
Article Snippet: .. Cell pellets were resuspended in 50 μL of buffer containing a mixture of fluorochrome-conjugated, oligo-conjugated antibodies (Supplemental Table 1) and fixable viability dye eFluor 506 (ThermoFisher Scientific) incubated for 25 minutes on ice. .. Cell sorting was performed using an Aria II cell sorter (BD Biosciences), excluding dead cells, doublets, and debris while selecting specific cell types for further analysis.

Review